Journal: Cell Biomaterials

Article Title: Desmoplastic tumor priming using clinical-stage corticosteroid liposomes

doi: 10.1016/j.celbio.2025.100051

Figure Lengend Snippet: LipoDex priming improves the tumor accumulation and penetration of polymeric nanocarriers (A) Top: whole-body CT-FMT images showing longitudinal PHPMA biodistribution and tumor accumulation in MLS tumor-bearing mice. Bottom panel: CT-segmented tumor slices (in green) exemplify FMT-assessed polymer accumulation (color-coded clouds). (B) Normalized PHPMA tumor accumulation quantification in control vs. LipoDex-treated mice, exemplifying that the 2.5 mg/kg dose of the liposomal GC improves polymer tumor targeting. (C) Fluorescence microscopy analysis of polymer extravasation and penetration out of lectin-stained tumor blood vessels into the interstitium. (D) Quantification of PHPMA intratumoral distribution reveals that all LipoDex doses enhance polymer penetration as compared to the saline-treated controls. Values represent average ± SD. One-way ANOVA with Tukey’s multiple comparisons test was performed for statistical analysis. ns > 0.05, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. Scale bar represents 50 μm.

Article Snippet: Right after the last therapy injection (Doxil or saline), mice were subjected to whole-body CT scan (U-CT OI, MILabs B.V., a Rigaku Company, Utrecht, the Netherlands) under anesthesia (2% isoflurane).

Techniques: Polymer, Control, Fluorescence, Microscopy, Staining, Saline

Journal: Cell Biomaterials

Article Title: Desmoplastic tumor priming using clinical-stage corticosteroid liposomes

doi: 10.1016/j.celbio.2025.100051

Figure Lengend Snippet: LipoDex priming improves the tumor accumulation and penetration of liposomal nanocarriers (A) Top: whole-body CT-FLT imaging exemplifies PEGylated liposome biodistribution and tumor accumulation in untreated, free Dex-treated, and LipoDex-treated mice. Bottom panel: CT-segmented tumor slices (green) show liposome tumor accumulation (color-coded clouds). (B) Quantification of liposome tumor accumulation demonstrates significant enhancement in overall tumor accumulation upon LipoDex priming compared to free Dex treatment and saline controls. (C) Fluorescence microscopy images depict extravasation and penetration of liposomes out of lectin-labeled tumor blood vessels into the interstitium. (D) Quantification of intratumoral distribution reveals that LipoDex priming significantly enhances liposome penetration into deeper tumor compartments. Values represent average ± SD. One-way ANOVA with Tukey’s multiple comparisons test was performed. ns > 0.05, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. Scale bar represents 50 μm.

Article Snippet: Right after the last therapy injection (Doxil or saline), mice were subjected to whole-body CT scan (U-CT OI, MILabs B.V., a Rigaku Company, Utrecht, the Netherlands) under anesthesia (2% isoflurane).

Techniques: Imaging, Saline, Fluorescence, Microscopy, Liposomes, Labeling

Journal: Materials

Article Title: Analysis of Geometrical Accuracy and Surface Quality of Threaded and Spline Connections Manufactured Using MEX, MJ and VAT Additive Technologies

doi: 10.3390/ma17215143

Figure Lengend Snippet: Measurement setup equipped with the ATOS Triple Scan II Blue Light 3D scanner.

Article Snippet: Geometric accuracy measurements of the produced spline connection components were carried out using the ATOS Triple Scan II Blue Light optical 3D scanner (Zeiss, Oberkochen, Germany) presented in .

Techniques:

Journal: Materials

Article Title: Analysis of Geometrical Accuracy and Surface Quality of Threaded and Spline Connections Manufactured Using MEX, MJ and VAT Additive Technologies

doi: 10.3390/ma17215143

Figure Lengend Snippet: 3D ATOS Triple Scan II Blue Light parameters.

Article Snippet: Geometric accuracy measurements of the produced spline connection components were carried out using the ATOS Triple Scan II Blue Light optical 3D scanner (Zeiss, Oberkochen, Germany) presented in .

Techniques:

Journal: Molecular Biomedicine

Article Title: Multimodality molecular imaging of the alveolar-capillary barrier in lung disease using albumin based optical and PET tracers

doi: 10.1186/s43556-020-00020-1

Figure Lengend Snippet: 3D OI/CT detection of LPS-induced lung inflammation using Cy7-albumin. a representative coronal images of Cy7-albumin uptake in the lungs of the PBS (top, n = 4) and LPS (bottom, n = 5) treated mice 24 h post injection. CT (left panels) and fusion 3D OI/CT (right panels) are shown. Note that infiltrates visible on CT (red arrows) are co-localized with Cy7-albumin accumulation detected by the 3D optical scan. b Cy7-albumin is detected in dissected lungs of mice treated with LPS ( n = 5) or PBS ( n = 4, control). *, p < 0.01

Article Snippet: Immediately after 3D optical /CT scan, lungs were dissected, placed in 4% PFA in a 6-well plate, and imaged using the MILabs 3D optical scanner.

Techniques: Injection, Control

Journal: Cancers

Article Title: Inhibition of HIF-1α by Atorvastatin During 131 I-RTX Therapy in Burkitt’s Lymphoma Model

doi: 10.3390/cancers12051203

Figure Lengend Snippet: In vivo images of 131 I-RTX after ATV treatment in Raji lymphoma tumor tissue ( a ) Representative SPECT/CT images of Raji-xenografted mice were acquired at 2, 24, 48, and 72 h after injection of 131 I-RTX (upper row) and ATV plus 131 I-RTX (lower row). White dotted circles indicate the tumor region. ( b ) The quantification of 131 I-RTX accumulation in tumors is represented by the tumor to blood ratio at each time point (* p < 0.05). The data are the mean ± SD from five independent mice. ( c ) Autoradiography of 131 I-RTX in Raji tumors was conducted after the acquisition of SPECT images (upper row). ( d ) The total accumulation of 131 I-RTX per tumor tissue (** p < 0.005). The data are the mean ± SD from ten independent images. ( e ) IVIS images presented a lower signal for combination group of ATV plus 131 I-RTX (lower row) than the 131 I-RTX alone group (upper row) in Raji-luciferase cell xenografted orthotropic model. ( f ) Total number of photons per second was 1.42 × 107 for 131 I-RTX and 3.84 × 107 for 131 I-RTX + ATV ( p * < 0.05). ( g ) The result of effective dose for 131 I-RTX and 131 I-RTX+ ATV.

Article Snippet: For in vivo optical imaging, scans using IVIS Spectrum (Perkinelmer, Waltham, MA, USA) were performed 14 days after the cell injection.

Techniques: In Vivo, Single Photon Emission Computed Tomography, Injection, Autoradiography, Luciferase